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The majority of polycyclic aromatic hydrocarbons (PAHs) are environmentally persistent and toxic to both plant and animal.However, understanding stress responses as well as strengthening plant tolerance and detoxification systems is important for plant based remediation of PAHs.Here, we studied the effects of 24-epibrassinolide (EBL) on phenanthrene (PHE)-a three-ringed PAH-induced stress in tomato plant.Twenty-one-day PHE exposure induced oxidative stress and caused significant biomass reduction.Obvious ultrastructural alterations characterized by irregular shaped nucleus, fewer mitochondria, thin cell wall and high vacuolation were observed in PHE treated root tip cells.Increased malondialdehyde (MDA) and hydrogen peroxide (H2O2) content confirmed PHE-induced oxidative damage in leaves.