【摘 要】
：
In order to establish DNA fingerprinting to identify the purity of cabbage hybrid,the genomic DNAs of 4 hybrids(Xiaguang,Zaoxia 16,Zhengchun,Hanguang 2)and its parents were used as group of template D
【机 构】
：
Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,P.R.China
【出 处】
：
第十九届国际十字花科遗传学会及芸薹属2014年年会
论文部分内容阅读
In order to establish DNA fingerprinting to identify the purity of cabbage hybrid,the genomic DNAs of 4 hybrids(Xiaguang,Zaoxia 16,Zhengchun,Hanguang 2)and its parents were used as group of template DNA respectively.In 4 groups,3 efficacious primers were gained in each group,they were S15,S42,S147 and S42,S78,S88 and S42,S103,S193 and S42,S89,S151 respectively.The specific RAPD fingerprints in the 4 groups could be established with S42 primer and then transformed into digital fingerprinting.BSA method was applied to study the immature-bolting characteristic and find its linkage RAPD markers.Through selecting of 260 RAPD primers,we gained 4 primers,which can amplicate markers linked to the immature-bolting gene of cabbage.There were 5 linked markers showed from the relationship analysis result of F2 population with the 4 primers,and the linkage distances of the markers are different,the closest one is 8.58 cM.Three markers that were closest to immature-bolting gene correspondingly were examined of sequence and named S97-392,U16-446,and U16-562 according to fragment size and primer source.The result of sequence comparison between the 3 markers showed that there was little comparability,and no same DNA fragment or gene in GeneBank.
其他文献
Twenty oilseed Brassica breeding populations derived from mass selection or inter-specific hybridization were field screened for resistance to three separate isolates of Sclerotinia sclerotiorum,the c
Verticillium longisporum is a vascular pathogen infecting oilseed rape.Quantitative disease resistance (QDR) is the major control means against this disease but poorly understood.To study QDR against
Pyramiding resistance genes is an established breeding method for producing Brassica cultivars with durable and broad spectrum resistance to clubroot disease caused by Plasmodiophora brassicae.Identif
Glucosinolates (GLS) are a major class of secondary metabolites found in the Brassicaceae family,which includes important Brassica crops and the model plant Arabidopsis thaliana.The pattern of synthes
Plants experience sudden increase in salinity after transplantation into salt contaminate field.The salt responsive transcriptome of a relatively salt tolerant Brassica napus line,N119,identified in o
Methods for detection of quantitative resistance against Leptosphaeria maculans in Brassica napus were investigated in controlled environments using doubled haploid (DH) lines A30 (susceptible) and C1
Canola (Brassica napus L.) is an important oilseed crop globally,but vulnerable to heat and drought stress,especially at the early reproductive stage.In this presentation,12 Brassica genotypes,includi
Oilseed rape (Brassica napus L.,genome AACC,2n = 38) has a narrow genetic base and suffers heavy yield losses caused by Sclerotinia sclerotiorum.In this study we used a hexaploidisation strategy to di
Most studies of screening waterlogging tolerance were performed at a specific development stage,with the implicit assumption that differences in waterlogging tolerance expressed in these systems will
Raphanus sativus L.could be interesting as source for introgressions of resistance genes against different pathogenes,but also against abiotic stress.A complete set (a-i) of disomic rapeseed-radish ch