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Background: The mulberry silkworm, Bombyx mori, is renowned for important economic valuable for silk production as well as being a lepidoptera model insect for physiology, genetics and biotechnology research.The posterior silk gland (PSG) is a highly specialized organ and responsible for the synthesis and secretion of silk core fibroin proteins.Although a number of functional genomics and proteomics has been performed in the Bombyx PSG, the mechanisms that regulate biosynthesis of silk protein remains to be further elucidated.