Improvement of Proteome Coverage by combining the Precursor Ion Exclusion and Inclusion MS /MS Data

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Data-dependent acquisition (DDA) precursor ion selection,the most common used method in LC-MS/MS for complex samples such as complete proteomes,is dependent on the signal intensity of precursor ions detected in a survey scan.Limited sequencing speed of current MS instruments is incapable of analyzing all precursor ions in every survey scan.
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