Antimony induces G2/M cell cycle arrest in A549 cells in a ROSdependent manner

• 作 者: Wenpan Fang Xinyuan Zhao Chen Lin Guangdi Chen Zhengping Xu
• 机 构: Bioelectromagnetics Laboratory,Zhejiang University School of Medicine,Hangzhou,310058;Institute of E
• 出 处: 中国生物化学与分子生物学会2016年全国学术会议
• 发表日期: 2016年3期
• 关键词: Antimony ROS Cell cycle Respiratory system

　　Antimony(Sb)is a metalloid that has been widely exploited in industry,such as the production of flame retardant,alloy materials,sliding bearings and solder.Due to mining and smelting processes,large quantities of Sb have been released and resulted in serious Sb contamination of our working and living environment.Furthermore,coal combustion and Sbassociated products consumed in daily life are also important Sb contamination sources.Environmental Sb exposure may cause respiratory irritation,pneumoconiosis,spotting on the skin and gastrointestinal symptoms.In addition,antimony trioxide is possibly carcinogenic to humans.However,its toxicological mechanisms on respiratory system remains unknown and is of great concern.In our present work,the human lung adenocarcinoma cell line(A549 cells)were exposed to different concentrations of SbCl3 for 24 hours which simulates respiratory system exposure of antimony in the occupational environment.The result of CCK8-based viability assay indicated that a concentration of 200 μm SbCl3 caused considerable decrease in cell viability,but Annexin Ⅴ detection showed no significant cells apoptosis.Further,cell cycle arrest was observed at G2/M phase accompanied with an increase of ROS level.Moreover,Sb-induced cell cycle arrest was greatly ameliorated when ROS was scavenged by the antioxidant N-acetylcysteine(NAC),and the cell viability was improved as well in Sb and NAC co-treated cells.These data demonstrate that Sb induces cell cycle arrest of A549 cells in a ROS-dependent manner,which may shed light on the toxicity of antimony and developing prevention measures towards occupational exposure.