【摘 要】
：
Silybum marianum was a crop with medicinal value and ornamental value which effective components was silymarin.In this article,11 experimental samples were collected from Zhejiang,Jilin,Neimenggu,Liao
【机 构】
：
College of Life Sciences,Zhejiang Sci-Tech University,Hangzhou,310018
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
Silybum marianum was a crop with medicinal value and ornamental value which effective components was silymarin.In this article,11 experimental samples were collected from Zhejiang,Jilin,Neimenggu,Liaoning and Heilongjiang province.DNA barcode technology was used to identify natural medicines,and we finded that the ITS2 sequence more suitable for silybum marianum identification.In this study,an effective pressurized water extraction and HPLC methods were developed for simultaneous determination of 8 compounds including silymarin,silychristin,silydianin,silybinA,silybinB,Dehydrosilybin,isosilybinA and isosilybinB.All calibration curves showed good linearity(R2>0.996)within test ranges.The contents of silybin(A,B)are all more than 0.6%and conformed the demand of Chinese Pharmacopoeia(CP).The highest level of silymarin sample come from Haicheng,Liaoning province,reached 4.073%.The average content of silychristin was 0.063%and the average content of silydianin was 0.035%.
其他文献
Atherosclerosis(AS)is an important pathological basis of cardiovascular events,and damage caused by AS is an important factor causing cardiovascular disease and death.In recent years,the study of the
Gene defects have been recognized as prominent factors in the etiology and pathogenesis of neurodegenerations.Among 60 neurodegeneration-related mutations in progranulin(PGRN),the mutation in PGRN gen
Ultrafine particles(UFPs)are capable of inducing pulmonary fibrosis(PF);however,the underlying molecular mechanisms have not been fully elucidated.Here,we reported an important role of autophagy in th
Cysteine-rich secretory proteins(CRISPs),characterized by 16 conserved cysteines,are distributed in a wide range of organisms.In the previous studies,a novel CRISP family member(cysteine-rich buccal g
Background & Aims: Colorectal cancer(CRC)is the third most commonly diagnosed cancer in males and the second in females.The branched-chain a-keto acid dehydrogenase kinase(BCKDK)is a rate-limiting enz
蛹虫草(Cordyceps militaris)中的SOD 酶可抑制人体内过氧化脂质(LPO)的生成和清除衰老产物(氧化自由基)，其功效已由试验证明超过了传统的抗氧化中药。此外，蛹虫草中的虫草多糖、蛋白质、虫草酸都具有较强的的抗氧化性。生物电网络系统是生物信号传递途径之一，研究发现，人体的皮肤上具有一些特殊电学特性的穴位点与中医传统针灸的穴位不谋而合，这些点具有低阻抗、高电容、特异性强、传导速度快
Previously,we have observed that all the n-3 PUFAs,especially DHA and EPA,were markedly decreased in the highfat diet(HFD)and low-dose streptozotocin-(STZ)induced type 2 diabetic rat heart.In the pres
间质细胞代谢重编程在调节肿瘤恶性进展过程中发挥重要作用。而结肠癌相关巨噬细胞(Tumor associatedmacrophage,TAM)的代谢活性尚未阐明。本研究将揭示结肠癌TAM来源的代谢因子及其在结肠癌恶性进展中的作用及机制。我们率先从细胞、动物实验及人体标本中证实：结肠癌可诱导TAM中ABHD5表达升高。我们成功构建了巨噬细胞特异的ABHD5转基因(TgABHD5)小鼠,并证实TAM中A
目的：在长程高脂膳食诱导的脂肪肝小鼠中,探讨白介素-17A(Interleukin 17A,IL-17A)基因缺陷对LPS 诱导的急性肝损伤中的作用及其机制.方法：采用正常膳食及长程高脂膳食(HF)喂养野生型C57BL/6 小鼠(n=10)及相同遗传背景的IL-17A 基因敲除小鼠(n=6),6 个月后,辅之以腹腔一次性注射LPS(4mg/kg 体重),注射后16 小时取血清并处死动物,通过检测血
This work aims to investigate the role of the loopβ3αC amino acids in the structure and function of isocitrate dehydrogenase kinase/phosphatase(AceK).The results demonstrate that the precise configura