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N-methyl-D-asp.artate receptor(NMDA receptors) play a critical role in learning and memory.NR2A and NR2B subunits are predominant to form the NMDA receptor complex with NR1 subunit in the forebrain.It has been reported that the up-regulation of NR2B and the higher ratio of NR2B/NR2A resulted in prolonged opening of NMDARs and enhanced long-term potentiation in hippocampus.In addition,the NR2B transgenic mice have better performances in the cognitive behavioral tasks.However,there is little study about the effect of up-regulation of NR2A subunits and the lower ratio of NR2B/NR2A on the synaptic plasticity and memory.In present study,using brain-slice in vitro electrophysiological and behavioral methods,we investigated the NR2A transgenic mice,in which NR2A subunits specifically overexpressed in the forebrain,to detect the effect of NR2A up-regulation in forebain on amygadala synaptic plasticity and amygadala-related fear memory.Result:1) NMDA-receptor-mediated LTP in transgenic mice is significantly smaller than those in wild-type littermates,indicating that the overexpression of NR 2A in forbrain impaired LTP in amygadala.