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目的:优选连参颗粒的提取工艺条件,为该制剂的开发提供参考。方法:以挥发油得率为指标,采用单因素试验考察提取时间、浸泡时间、加水量对当归、辛夷、肉桂中挥发油提取工艺的影响;采用UV测定总生物碱含量,检测波长350 nm;运用HPLC测定盐酸小檗碱含量,流动相乙腈-0.05 mol·L-1磷酸二氢钾溶液(50∶50),检测波长345 nm。以总生物碱、盐酸小檗碱含量及干膏率的综合评分为指标,采用L9(34)正交试验考察加水量、提取时间、提取次数对连参颗粒水提取工艺的影响。结果:挥发油提取工艺为加8倍量水浸泡1.0 h,利用水蒸气蒸馏提取5.0 h;最佳水提工艺为加8倍量水提取3次,每次1.0h;干膏率、总生物碱及盐酸小檗碱提取量分别为25.91%,512.34 mg,130.81 mg。结论:该提取工艺稳定可行,适用于连参颗粒的工业化生产。
OBJECTIVE: To optimize the extraction process conditions for the ginseng granules and provide a reference for the development of the preparation. Methods: The volatile oil yield was used as an index to study the effects of extraction time, soaking time and water addition on the extraction of volatile oil from Angelica sinensis, Magnolia biondii and Cinnamon. The total alkaloid content was determined by UV detection at 350 nm. The content of berberine hydrochloride was determined. The mobile phase was acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution (50:50). The detection wavelength was 345 nm. The total alkaloid, berberine content and dry cream rate of the composite score as an indicator, using L9 (34) orthogonal test to examine water, extraction time, the number of extraction on the water extraction process Lianzhang particles. Results: The extraction process of essential oil was 1.0 h with 8 times the amount of water soaked for 5.0 h by steam distillation. The best water extraction process was 8 times of water extraction for 3 times, each time was 1.0 h. The dry extract rate, total alkaloids And berberine hydrochloride were 25.91%, 512.34 mg and 130.81 mg, respectively. Conclusion: The extraction process is stable and feasible, suitable for the industrial production of pellets.