WhetheriNaYF4:Yb3+,Er3+@NaGdF4@TaOxcanbeusedtoeffectivelylabelmesenchymalstemcells(MSCs)

• 作 者: yangqu xiaofeng taosong taoai
• 机 构: 上海交通大学医学院附属第九人民医院
• 出 处: 中华放射学学术大会2016、中华医学会第23次全国放射学学术大会暨中华医学会第24次全国影像技术学术大会
• 发表日期: 2016年5期
• 关键词: dual-energy CT Mesenchymal stemcells nanoparticle bonerepair

　　Objective: TodeterminewhetheriNaYF4:Yb3+,Er3+@NaGdF4@TaOxcanbeusedtoeffectivelylabelmesenchymalstemcells(MSCs)invivoandcanbeusedfortrackingofstemcelltransplantsbydual-energyCTintissueengineeringandbonerepair. Methods:Thisstudywasapprovedbytheinstitutionalanimalcareandusecommittee.Mesenchymalstemcells(MSCs)was extractedfrombonemarrowofNewZealandRabbits(8weeksold).iNaYF4:Yb3+,Er3+@NaGdF4@TaOxnanoparticleswith1mg/ml wastransfectedintoMesenchymalstemcells(MSCs)bylipofectamine.UtilizationofCCK-8detectionnanoparticlesoncell viabilityofMSCs.MeasuringtheeffectofnanoparticlesonMSCsapoptosisandcellcyclebyflowcytometry.Andtheeffectof nanoparticlesonosteogenic,chondrogenicandadipogenicdifferentiationwasdetected.Theuseoftwo-photonlaserscanning confocaldetectionnanoparticlesenterMSCs.Usingthedual-energyCTdetectedthenanoparticlesinMSCsatdifferenttime pointinvitro.Thenthecompositeofβ-TCPandMSCswhichtransfectedwithnanoparticleswasimplantedintothebonedefect, anddetectedbydual-energyCTatdifferenttimepoint. Result: wefoundthat iNaYF4:Yb3+,Er3+@NaGdF4@TaOxnanoparticleshadnoeffectonMesenchymalstemcells(MSCs)cell viability,apoptosisandcellcycle,Ontheotherhandtheosteogenic,chondrogenicandadipogenicdifferentiationabilityof Mesenchymalstemcells(MSCs)hadaffectedbyiNaYF4:Yb3+,Er3+@NaGdF4@TaOxnanoparticles.Wehadobservedalotof nanoparticleswastransfectedintotheMSCsbytwo-photonlaserscanningconfocal.Strongsignalwasdetectedbydual-eneryCT invitroandinvivo,butthesignaldecreasingwithtime. Conclusion: iNaYF4:Yb3+,Er3+@NaGdF4@TaOxnanoparticlesisansafematerialsforlabelingMSCs. iNaYF4:Yb3+,Er3+@NaGdF4@TaOxnanoparticlescanbeusedtoeffectivelylabelMSCsinvivoandcanbeusedfortrackingof stemcelltransplantswithCTimaging.