Induction and mechanism of HeLa cell apoptosis by 9-Oxo-10, 11-dehydroageraphorone from Eupatorium a

• 作 者: LIAOFei[1]HUYanchun[2]WULei[2]TANHui[2]LUOBiao[2]LUOLijuan[2]HEYajun[2]QIAOYan[2]MOQuan[2]ZHOUYancheng[2]ZUOZhicai[2]RENZhihua[2]DENGJunliang[2]WEIYahui[3]
• 机 构: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicin
• 出 处: 中国畜牧兽医学会家畜内科学会2014年学术研讨会
• 发表日期: 2014年期

　　9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from Eupatorium adenophorum.The present study aims to explore the induction and mechanism of HeLa cell apoptosis by 9-Oxo-10,11-dehydroageraphorone.The apoptosis inducing effect of the euptox A on HeLa cells was examined by MTT assay.Meanwhile, the mechanism was analyzed by flow cytometry and real-time PCR.Flow cytometry results suggest that euptox A could effectively inhibit the proliferation of HeLa cells, arrest the cell cycle transition from S to G2/M phase, cant continue to complete the cell cycle activity (mainly form four times and mitosis), and inake the cell proliferation, the QRT-PCR detection results showed that euptox A could induce apoptosis through improving the gene expression level of apoptosis proteases such as caspase-10 in HeLa cells.Its mechanism of action was probably associated with up-regulating apoptotic gene expression and arresting the cell cycle.