【摘 要】
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Introduction Microfluidic-based single-cell trapping in vitro is an emerging approach for the study of single cellular biological behaviors in response to the microenvironment.Most of the existing mic
【机 构】
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Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering,
【出 处】
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2016年大连国际微纳流体和微流控芯片大会(2016 International Conference of Micro
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Introduction Microfluidic-based single-cell trapping in vitro is an emerging approach for the study of single cellular biological behaviors in response to the microenvironment.Most of the existing microfluidic-based methods for single-cell trapping commonly have relied on stagnation point flows or physical barriers.The trapping method with physical barriers could capture a large numbers of single cells with high efficiency, but would fail to easily load external dynamic biochemical signals for the trapped cells.Another trapping method by stagnation point flows was able to transport biochemical signals but had low stability.The introduction with feedback controller may improve the tapping stability, but would need more external control equipment.Thus a microfluidic device for single-cell trapping with high stability, inexpensive, and easy-to-use to quantitatively load dynamic biochemical stimuli, is necessary.
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