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目的 The expression and diagnosis significance of GAS5 in the plasma of the early stage of NSCLC patients was unknown.方法 We extracted the free RNA in the plasma and analyzed the expression of GAS5 with qRT-PCR.Suitable statistics methods were used to compare the plasma GAS5 levels of preoperative and postoperative plasma samples between the NSCLC patients and healthy controls.ROC analysis was used to evaluate the diagnostic sensitivity and specificity of plasma GAS5 in NSCLC.结果 The results showed that GAS5 was detectable and stable in the plasma of NSCLC patients.Furthermore,the plasma levels of GAS5 were significantly down-regulation in NSCLC patients compared with healthy controls(P=0.000).Moreover,GAS5 levels increased markedly on the seventh day after surgery compared with preoperative GAS5 levels in NSCLC patients(P=0.003).GAS5 expression levels could be used to distinguish NSCLC patients from control patients with an area under the curve of 0.832(P＜0.0001,sensitivity,82.2％,specificity,72.7％).The combination of the GAS5 and CEA could produce an area of 0.909 under the receiver operating characteristic curve in distinguishing NSCLC patients from control subjects(95％CI 0.857-0.962,P=0.000).结论 GAS5 could be an early ideal biomarker for the diagnosis of NSCLC.