【摘 要】
：
1.Background: Automated identification and cytometric quantification of molecular markers (antibodies, histological stains, genetic probes) on the single cell level in tissue sections by means of slid
【机 构】
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TissueGnostics GmbH Austria
【出 处】
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BITs 3rd Annual World Cancer Congress-2012(2012第五届世界癌症大会)
论文部分内容阅读
1.Background: Automated identification and cytometric quantification of molecular markers (antibodies, histological stains, genetic probes) on the single cell level in tissue sections by means of slide-based cytometry has become an essential tool in biomedical research and routine analysis in histopathology.TissueFAXS is the microscopic equivalent to flow cytometry-applicable to tissue sections and cell culture monolayers and allows observer independent analysis of all types of cells in all kinds of tissue sections in-situ.2.Technollogy and Method: The TissueFAXS system consists of a high-end motorized fluorescence microscope and sophisticated software for image analysis.The samples can be stained by means of immunohistochemistry or immunofluorescence.By using the nuclear marker (e.g.hematoxilin or DAPI) the location of the individual cells is identified and the system quantifies the staining intensity in each color channel for each and every cell.It allows even to distinguish between and compare between nuclear and cytoplasmatic marker expression in the same sample.Representation of cellular parameters may be obtained by dot plots in a FACS-like manner.3.Results: Results presented come from several studies and comprise different applications of slide-based cytometry.(i) Phenotypic characterization of tissue infiltrating leukocytes in tumor biology, transplantation immunology and autoimmune diseases exemplifying the quantification of markers CD1a, CD3, CD4, CD8, CD1 1c, and CD68 in renal cell carcinoma, renal allograft rejection and atopic dermatitis.(ii) Quantification of proliferation marker Ki67 and Her2 in breast cancer.4.Discussion: The data above demonstrate novel approaches in research and improved data transparency in diagnosis using slide-based cytometry.Observer-biased visual estimation in immunohistological analysis of tissue samples is replaced by observer independent measurements on the single-cell level, which is especially important in multi-center studies and set-up of clinical studies for the pharmaceutical industry.
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