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Graphene oxide (GO),with a mass of oxygen groups,shows excellent processibility and stability in aqueous solution [1],specifically,it shows up to be nagetively charged in basic solution.Despite recent progress of Ruthenium ( Ⅱ ) complex with GO for sensing application due to their strong electrochemiluminescence (ECL) signal [2],the detailed study of the interactions between GO and ruthenium complex remains to be elucidated.