【摘 要】
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Hematopoietic stem/progenitor cell(HSPC)mobilization from the bone marrow to the bloodstream is a required step for blood cell renewal and HSPC motility is a clinically relevant standard for periphera
【机 构】
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School of Medicine and Life Sciences,Nanjing University of Chinese Medicine,138 Xianlin Ave,Nanjing
【出 处】
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中国化学会第十二届全国微全分析系统学术会议、第七届全国微纳尺度生物分离分析学术会议、第七届国际微流控学学术论坛
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Hematopoietic stem/progenitor cell(HSPC)mobilization from the bone marrow to the bloodstream is a required step for blood cell renewal and HSPC motility is a clinically relevant standard for peripheral blood stem cell transplantation.Individual HSPCs exhibit considerable heterogeneity in motility behaviors,which are subject to complex intrinsic and extrinsic regulatory mechanisms.Motility based cell sorting is then demanded to fulfill the study of such mechanism complexity.However,due to the HSPC heterogeneity and difficulty in monitoring cell motility,such a platform is still not available.With the recent development of microfluidics technology,motility-based monitoring,sorting,collecting,and analysis of HSPCs behaviors are highly possible and achievable if fluid channels and structures are correctly engineered.Here,we present a new design of microfluidic arrays for single-cell trapping,enabling high-throughput analysis of individual HSPC motility and behavior.Using these arrays,we observe that HSPC motility is positively correlated with CD34 asymmetric inheritance and cell differentiation.Transcriptomic analysis of HSPCs sorted according to motility reveals changes in expression of genes associated with the regulation of stem-cell maintenance.Ultimately,our novel physical cell-sorting system can facilitate the screening of HSPC mobilization compounds and the analysis of signals driving HSPC fate decisions.
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