Auxin Regulates Plant Stem Cells Homeostasis in Arabidopsis

来源 :12th International Conference on Plant Biology Frontiers(第十二 | 被引量 : 0次 | 上传用户:allen75
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  Stem-cell fate in the plant of Arabidopsis thaliana is controlled by a complex regulatory network of signaling molecules and transcription factors.The classic phytohormones play essential roles in the maintenance of stem-cell systems, and exhibit complex functional interactions.In shoot apical meristem (SAM), auxin generated universally, and then transferred to the peripheral zone (PZ) for the lateral organs initiation.In addition, auxin can also be transferred into the central zone (CZ) where harbored plant stem cells.Previously we have shown that the AUXIN RESPONSE FACTOR5/ MONOPTEROS (MP) transcription factor, mediated the cross talk between auxin and cytokinin in stem cells.However, the biological functions of auxin in stem cells are still largely unknown.By using RNA sequencing, weidentified several new MP targets specifically expressed in stem cells.One of those targets,MTA1, can be repressed by MP, while knocking out MP cause transcriptional up regulation of MAT1.MAT1 is a transcription factor only functional in stem cells.Further analysis show that MTA1 is sufficient to active CL V3 expression in plant cells.These data suggested that auxin could regulate stem cells homeostasis via MP and MTA1 pathway.
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