Effect of pH in hydrophobic charge induction chromatography:Molecular dynamics simulation and experi

• 作 者: LinZhang;GuofengZhao;YanSun;
• 机 构: Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin Universi
• 出 处: The 5th Sino-US joint Conference of Chemical Engineering(第五届
• 发表日期: 2009年期

　　Low pH is usually required in hydrophobic charge rnduction chromatography (HCIC) for the facile elution, because this process is driven by the electrostatic repulsion between the protein and ligands carrying both positive charges.However,low pH is considered to be harmful for the native conformation of protein.