A further exploration of the impact of antinuclear antibodies on in vitro fertilization-embryo trans

• 作 者: YingYing;Yi-pingZhong;Can-quanZhou;Yan-wenXu;Chen-huiDing;QiongWang;JieLi;Xiao-tingShen;
• 机 构: Reproductive Medicine Center,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,C
• 出 处: 2013年粤桂妇产科学学术交流会议暨2013年广东省医学会妇产科学学术年会
• 发表日期: 2013年期

　　Objective: To determine the presences of antinuclear antibodies (ANA) in follicular fluid and embryos as to their potential impact on IVF outcome.Methods: A total of 100 women presenting to IVF/ICSI program were recruited in this study,wherein 50 women seropositive for ANA served as study group (ANA+ group) and 50 women seronegative for ANA served as control group (ANA-group),patients were age-matched in the two groups.ANA level in serum and follicular fluid were examined by ELISA assay and immunofluorescence assay was employed to detect IgG within embryos.Results: In ANA+ group,36 women showed positive ANA both in serum and follicular fluid (FF) on the day of ovum pick-up (OPU),wherein 34 exhibited fluorescence of IgG in embryos.All the 50 women in control group showed negative results in serum and FF ANA on OPU day and in embryo immunofluorescence.Serum ANA level positively correlated with FF ANA level.IVF outcomes were significantly poorer in ANA+ group,mainly reflecting in the less number of high-quality embryos,reduced rates of pregnancy,clinical pregnancy and implantation.Conclusion: The present data demonstrated the existence of ANA in follicular fluid and embryos,which may exert detrimental impact on IVF outcome.