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Yellow catfish(Pelteobagrus fulvidraco)is one of the most important freshwater aquaculture species in China.However,its small size and lower meat yield limit its edible value.Myostatin(MSTN)is a negative regulator of mammalian muscle growth.But,the function of Mstn in fish remains elusive.To explore roles of mstn gene in fish growth and create a strain of yellow catfish with high amount of muscle mass,we performed targeted disruption of mstn in yellow catfish using engineered zinc-finger nucleases (ZFNs).Employing zebrafish embryos as a screening system to identify ZFN actMty,we obtained one pair of ZFNs that can edit mstn in yellow catfish genome.Using the ZFNs,we successfully obtained two founders(Founder July29-7 and Founder July29-8)carrying mutated mstn gene in their germ cells.The mutated mstn allele inherited from Founder July29-7 was a null allele (mstnnju6)containing a 4 bp insertion,predicted to encode function null Mstn.The mutated mstn inherited from Founder July29-8 was a complex type of mutation(mstnnju7),predicted to encode a protein lacking two amino acids in the N-terminal secretory signal of Mstn.Totally,we obtained 6 mstnnju6+ and 14 mstnnju7/+ yellow catfish.To our best knowledge,this is the first endogenous gene knockout in aquaculture fish.Our result will help in understanding the roles of mstn gene in fish.