【摘 要】
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Purpose:This study is to investigate whether the neuroleptic agent pimozide inhibits stem-like cell maintenance and tumorigenicity in hepatocellular carcinoma (HCC).Method:Cell proliferation was measu
【机 构】
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Organ Transplantation Center,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 51063
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Purpose:This study is to investigate whether the neuroleptic agent pimozide inhibits stem-like cell maintenance and tumorigenicity in hepatocellular carcinoma (HCC).Method:Cell proliferation was measured by MTT assay and colony formation assay;self-renewal capacity of cells was detected by in vitro sphere formation assay or in vivo xenograft tumor animal model.Using the flow cytometry analysis,Ki67 and ALDH1 expression was determined,and distribution of cell cycle was analyzed by propidium iodide staining;Western-blotting assay was used to detect protein expression level of the stemnness transcription factors (Oct4,Nanog,Sox2 and Bmil) and the STAT3 signaling;CD133+ MHCC97L cells were isolated by magnetic bead cell sorting.The potentials of CD133+ cells for colony formation and tumorigenicity were evaluated by cloning and tumor formation following nude mice inoculation.
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