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The specificity of acupuncture points (acupoints) is one of the key concepts in traditional acupuncture theory.Laterality of the acupoint is an important aspect of acupoint specificity.In this study, a total of eighteen healthy female volunteers were recruited for this study.Electrocardiograms were recorded and heart rate variability was analyzed before, during and after PC6 was stimulated on either the left or right side.The results show that during acupuncture there were significant differences in the standard deviation of RR intervals (STDRR), root mean square of successive differences between RR intervals (RMSSD) and total power between the left PC6 stimulation group and the right PC6 stimulation group, which indicates that PC6 may have laterality.