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An ultrasensitive electrochemiluminescence (ECL)-based DNA assay for the detection of genetic code of H7N9 avian influenza virus (AVI) was developed by integrating a novel ECL quencher of graphitic carbon nitride (C3N4) nanosheet-supported cobalt porphyrin (hemin[Co(Ⅱ)]) as highly efficient coreactant scavenger.