【摘 要】
：
目的：研究Au纳米颗粒的制备、PEI对金纳米粒的修饰及表征,并对其作为基因载体的体外生物学特性进行检测.为临床应用提供理论和实验依据.材料与方法：(1)Au纳米颗粒的制备：采
【机 构】
：
东南大学医学院,江苏省生物材料和器件重点实验室
【出 处】
：
2013全国生物与医学纳米技术博士生学术论坛
论文部分内容阅读
目的：研究Au纳米颗粒的制备、PEI对金纳米粒的修饰及表征,并对其作为基因载体的体外生物学特性进行检测.为临床应用提供理论和实验依据.材料与方法：(1)Au纳米颗粒的制备：采用单步还原法,以柠檬酸三钠作为还原剂和稳定剂制备Au纳米颗粒.取1％的HAuCl4水溶液lml加入97ml水中油浴加热沸腾,然后迅速加入2ml 1％的柠檬酸三钠水溶液保持沸腾15分钟即可得到Au纳米颗粒.(2)用PEI对Au纳米粒进行表面修饰：将10mlHAuCl4水溶液(浓度为0.01g/ml))与10mlPEI水溶液(浓度0.02g/ml)混合磁粒搅拌15min,然后加入1％的柠檬酸三钠2ml,继续搅拌一个小时.溶液变为紫红色后,透析去除游离的PEI,得到表面修饰过的PEI-Au.用透射电子显微镜(TEM),对Au和PEI-Au纳米颗粒进行表征.
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