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Blow flies(Diptera: Calliphoridae)are important insects to carrion decomposition and heterotrophic biomass conversion.While blow fly diversity has been recorded throughout many locations of the world,there is a need to better understand community variation within and across ecoregions in any given country.Additionally,recent studies have demonstrated that microbes may affect how,when and why flies colonize certain carrion resources,while flies also change the microbial communities of the resource in an unexplored ecological coupling.Given that blow flies directly affect carrion decomposition,they may be important biological mediators of microbial succession and thereby changing these communities through three primary pathways: 1)directly competing with microbes for the resource through feeding activity; 2)introducing antimicrobial compounds to the resource; 3)transporting exogenous microbial species to the resource in a way that shifts community composition and succession; or 4)some combination of these activities.In an effort to assess how blow fly communities varied with fly species-specific microbiome communities,we conducted a survey of blow flies and their associated internal microbiome communities in several ecoregions of the USA.In June 2013,blow flies were collected from nine sites within six ecoregions of the eastern USA(Central Appalachians,Ridge and Valley,Northern Piedmont,Piedmont,Blue Ridge Mountains,and Southwestern Appalachians),and in August 2013 from seven locations representing two ecoregions near Juneau,Alaska(Pacific Coastal Mountains and Coastal Western Hemlock-Sitka Spruce Forest).The most abundant blow fly taxa from the eastern USA were Phormia regina,Lucilia spp.,Calliphora spp.and Cynomya spp.,representing 90.9%,7.4%and 1.7%of Calliphoridae,respectively,while C.terraenovae was most abundant in Alaska.The internal microbiome communities were variable among ecoregions at the phyletic and family levels.In the eastern USA,P.regina microbiomes were dominated by Bacteriodetes while Firmicutes and Proteobacteria dominated C.terraenovae microbiomes in Alaska.These results confirm that ecoregion dictates local blow fly species diversity and abundance,while providing the first descriptions of the Calliphoridae internal microbiome community and how these communities vary with species across ecoregions.As molecular sequencing technologies continue to improve,postmortem microbiome communities and their interactions with necrophagous flies may become more important considerations for the discovery of novel and potentially transformative pathways of biomass waste conversion and protein production.