THE STUDY ON THE ACTIVITY SINGLE-CHAIN VARIABLE FRAGMENT ANTIBODY AGAINST AFLATOXIN B1

• 作 者: YuanyuanZhang;
• 机 构: Faculty of Food, HLJ August First Land Reclamation University, CHINA
• 出 处: 第十四届世界食品科技大会
• 发表日期: 2008年期

　　To prepare hybridoma cell line excreting monoclonal antibody against aflatoxin B 1 using B cell hybridoma technique.The variable regions of the heavy chain (VH) and light chain (VL) region genes of anti-AFB1 mAb were isolated by PCR and joined by a flexiable DNA linker as a single-chain Fv (scFv) DNA fagment.Using Phage Display technique,the scFv DNA fragment was cloned into a phagemid (pCANTAB5E) and transformed into competent ceils of Escherichia coli TG1,in the presence of helper phage M13K07,the scFv fusion protein was displayed on the surfaces of recombinant phages,constructing phage antibody library.