【摘 要】
：
Gene knockout (KO) mice produced by gene targeting methods are essential tools for investigating endogenous gene function.In such targeting methods, genetic mut
【机 构】
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LaboratoryAnimalResourceCenter,UniversityofTsukuba,Japan
【出 处】
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2014第十一届中国实验动物科学年会
论文部分内容阅读
Gene knockout (KO) mice produced by gene targeting methods are essential tools for investigating endogenous gene function.In such targeting methods, genetic mutations are introduced by spontaneous homologous recombination in mouse embryonic stem cells (mESc).Gene KO mice are generated by the production of germline chimeras derived from specific gene-mutated mESc.Although reliable, this method is laborious, costly, and time consuming.In contrast, genome editing with engineered DNA nucleases induces site-directed gene mutations by direct injection of DNA or RNA of site-specific engineered nucleases into fertilized oocytes.It has been reported that 3 kinds of engineered nucleases (ZFN, TALENs and CRISPR/Cas) are useful for producing knockout (KO) mice.Therefore, these new technologies are expected to be useful for producing gene-modified animals.
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