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Acid mine drainage (AMD) contains high concentration of metal ions, and it will be easily formed in the presence of Acidithiobacillus ferrooxidans(Li et al., 2009).Quorum sensing is a cell-to-cell communication system regulate many behavior of bacteria (Whitehead et al., 2001).In this study, we tried to control the AMD formation by an efficient quorum sensing inhibitor (OSI).