【摘 要】
：
Gaeuman-nomyces graminis causes serious wheat take-all disease.A biocontrol strain,Bacillus subtilis YB-81 has been used for controlling this disease.In order to raising the antagonistic effect, the g
【机 构】
：
Plant Protection Institute, Henan Academy of Agricultural Sciences, China IPM Key Laboratory in Nort
【出 处】
：
中国植物病理学会2014年学术年会
论文部分内容阅读
Gaeuman-nomyces graminis causes serious wheat take-all disease.A biocontrol strain,Bacillus subtilis YB-81 has been used for controlling this disease.In order to raising the antagonistic effect, the growth conditions and culture medium of biocontrol strain YB-81 were optimized based on the response surface methodology.By using the BGP medium, the optimal culture conditions of the biocontrol strain YB-81 were determined at temperature at 36℃, pH 8.0, speeding at 200 r/min, and the incubation time of 36h.The total numbers of colony was 188 ten millions/mL, improving by 72% , and the inhibition rate to Gaeumannomyces gramims was 87.2%, improving by 32.5% with the optimal growth conditions.The optimal fermentation condition of the biocontrol strain YB-81 successively improved the growth efficiency.The result provides a theoretical basis for the large scale production.
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