【摘 要】
：
次氯酸盐因其强氧化性,被广泛用作纺织、造纸工业中的漂白剂以及自来水、工业循环水、游泳池水和医院污水等水体的消毒剂.但水体中次氯酸盐的含量过高时,可能导致二次污
【机 构】
：
浙江大学微分析系统研究所310058
【出 处】
：
第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
论文部分内容阅读
次氯酸盐因其强氧化性,被广泛用作纺织、造纸工业中的漂白剂以及自来水、工业循环水、游泳池水和医院污水等水体的消毒剂.但水体中次氯酸盐的含量过高时,可能导致二次污染,对健康产生危害[1].因此检测水体中次氯酸盐具有明确的应用价值.次氯酸钠的强氧化性可使鲁米诺氧化激发而发光.化学发光法具有灵敏度高、简单、速度快、无需外加光源、可持续在线检测等优点,广泛应用于环境样品与生物样品的检测.但次氯酸钠与鲁米诺混合后在800ms后发光信号就达到最大值,常规的流动分析系统因通道死体积过大,难以实现对最强发光信号的检测[2].我们采用单螺旋构型的微流控芯片(芯片微通道体积约0.5μL)作为次氯酸钠-鲁米诺在线混合及发光检测池,克服了上述常规流动分析检验的缺陷[3].在与微流动分析技术联用后,实现了次氯酸钠的连续自动分析.本工作针对微流动分析进样量小、发光信号强度受限、不利于低浓度次氯酸钠检测的缺陷,首次报道以5-磺基水杨酸(5-SSA)为该发光反应的增敏剂,提高次氯酸钠-鲁米诺发光分析灵敏度的方法.研究提出5-磺基水杨酸与鲁米诺激发态结合导致化学发光信号增强的增敏机理(Fig.1)；并对系统各项参数进行优化,取得近10倍增敏效应.结果显示：在进样量仅2μL,5-磺基水杨酸用量100pmol时,次氯酸钠的线性响应范围0.6-10μM,相关系数0.9963；分析5μM次氯酸钠,RSD为2.0％(n=11)；LOD为59.2nM.将该微流动发光分析系统用于分析自来水、游泳池水等实际水样中次氯酸钠,并将结果与标准DPD分光光度分析法进行对照,结果一致.表明本系统具有操作简单、试剂试样耗量低、分析通量高(72h-1)、灵敏度高、检测限低等优点.
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