论文部分内容阅读
Background/Aims To study theexpression and clinical significance of serum soluble major histocompatibility complex class Ⅰ-related chain A/B (sMICA/B), and its correlation with percentage of CD4+, CD8+, and NK cells, Liver fibrosis screening test, and liver enzymes in alcoholic liver disease (ALD).Methods Hainan Li ALD patients (n =141) and healthy Li subjects (n =100) were enrolled for the study.Liver enzymes were measured using automatic biochemical analyzer and Liver fibrosis screening test was used to study the correlation.In addition, sMICA/B expression in serum and percentage of CD4+, CD8+, and NK cells were determined using ELISA and flow cytometry respectively.Results Liver fibrosis screening test results and liver enzymes concentration were significantly higher (bothp<0.01), whereas the expression of sMICA and sMICB was significantly indifferent (p>0.01) between ALD patients and healthy controls.However, percentage of CD4+, CD8+, and NK cells were statistically lower in ALD patients than in healthy controls.The Kendalls tau-b correlation coefficientfor sMICA and sMICB/sMICA and LV was 0.561 and 0.120 respectively (p<0.01).Pearson correlation coefficient of sMICA with the percentage of CD4+, CD8+%, and NK cells was-0.587,-0.525, and-0.232 respectively, whereas the coefficient of sMICB was-0.590,-0.554, and-0.292 respectively (p<0.01).Conclusion 1.Liver fibrosis screening test is an excellent non-invasive approach for the diagnosis of hepatic fibrosis and shows significant correlation with liver enzymes.2.sMICA and sMICB failed to assess the degree of hepatic fibrosis.3.Decreased percentage of CD4+, CD8+, and NK cells were attributed as one of the risk factors for ALD.