Glycyrrhizic acid promotes M1 macrophage polarization in murine bone marrow-derived macrophages asso

• 作 者: Yulong Mao Baikui Wang Xin Xu Wei Du Weifen Li Youming Wang
• 机 构: Key Laboratory of Animal Molecular Nutrition of Education of Ministry, College of Animal Science, Zh
• 出 处: 中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
• 发表日期: 2016年12期

　　The roots and rhizomes of Glycyrrhiza species (licorice) have been widely used as natural sweeteners and herbal medicines.The aim of this study is to investigate the effect of glycyrrhizic acid (GA) from licorice on macrophage polarization.Both phenotypic and functional activities ofmurine bone marrow-derived macrophages (BMDMs) treated by GA were assessed with flow cytometry, real-time fluorescence PCR, cytochemistry assay, western blotting and enzyme linked immunosorbent assay.Our results showed that GA obviously increased the cell surface expression of CD80, CD86 and MHCII molecules.Meanwhile, GA upregulated the expression of CCR7 and the production of TNF-α, IL-12, IL-6,and NO (the markers of classically activated (Mi1) macrophages), whereas it downregulated the expression of MR, Ym1,and Arg1 (the markers of alternatively activated (M2) macrophage).The functional tests showed that GA dramatically enhanced the uptake of FITC-dextran and E.coli K88 by BMDMs and markedly decreased the intracellular survival of E.coli K88 and S.typhimurium.Moreover, we demonstrated that JNK and NF-rκB activation are required for GA-induced NO and M1-related cytokines production, while ERK1/2 pathway exhibits a regulatory effect via induction of IL-10.Together, these findings indicated that GA promoted polarization of M1 macrophages and enhanced its phagocytosis and bactericidal capacity.The results expanded our knowledge about the role of GA in macrophage polarization.