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Objective: To investigate the status miR-155 and its targeting eNOS under the stimulation of glucose and ageBSA and to explore the effect of icariside Ⅱ(ICA Ⅱ)on the diabetic endothelial dysfunction of human cavernous endothelial cells(HCECs)by using the miR-155 pathway.Methods: Purified HCECs were divided into three groups:normal group + BSA(NC group),Glucose + Age-BSA group(DM group),ICA Ⅱ treatment group(DM + ICA Ⅱ group).Western blot to detect the expression of eNOS and RAGE protein expression;immunofluorescence assay to detect the NO production;real time PCR to detect the expression of miR-155 and eNOS.Overexpression of miR155 was performed in HCECs with or without ICA Ⅱ interference.Results: Under the diabetic induction,the expression of eNOS in DM group is significantly reduced compared with that in NC group and the expression of RAGE in DM group is significantly up-regulated compared with that in NC group(P<0.05),but the DM + ICA Ⅱ group showed higher eNOS expression and lower RAGE expression compared with those in the DM group.MiR-155 is higher after overexpression but can be repressed by ICA Ⅱ,eNOS expression was lower after overexpression but was increased by ICA Ⅱ.Conclusions: Under the glucose and Age-BSA stimulation,ICA Ⅱ may induce NO production in HCECs via the activation of eNOS by miR-155 so as to restore the HCECs functions.