【摘 要】
：
Modularized biological parts are imperative for the engineering of genetic systems.Therefore, as an engineering process to eliminate the widespread interact
【机 构】
：
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering,Institute of Microbiology,Ch
论文部分内容阅读
Modularized biological parts are imperative for the engineering of genetic systems.Therefore, as an engineering process to eliminate the widespread interactions between natural biological components, refinement is an urgent, substantial work.However, it is progressed at a slow pace.Here we developed a production line to refine promoters and operators, the major players of transcriptional control in prokaryotes.The line realized the functional decoupling of promoter core regions and operators in transcriptional control.As a result, we were able to develop a non-free parameter model to direct the manufacturing of transcriptional devices.As a proof, we exhaustively tested the combination of 6 promoters and 4 operator sets to biologically implement transcriptional devices.Further, 82 random combinations of 52 promoters and 35 operators were also tested.The results showed that the gene expression control using these devices is in high precision with the model prediction.
其他文献
每年二氧化碳排放量惊人2013年,全球二氧化碳排放量361亿吨,中国二氧化碳排放量100亿吨,是欧盟和美国排放量的总和,全球最大,碳排放、碳减排压力巨大.二氧化碳综合利用-难目
介绍内容项目背景产品市场项目背景产品市场什么是高阻隔塑料?阻湿性阻香性……阻气性项目背景产品市场中国每年支牙膏管只啤酒瓶各种食品包装膜阻隔的种类对O2、H2O、CO2和
Contents目录Why study on starch-based materials为什么要发展淀粉基材料Microstructures of starches淀粉的结构Starch-based on biodegradable polymers淀粉基可生物降解
生物塑料:生物质来源或可完全生物降解BioPlastics: Bio-based or Bio-degradable Bio-Plastics: BBP or BDP 生物塑料-BBP (Biomass-based): Reduce Carbon Foot Print .May
聚乳酸是一种生物基和生物降解高分子，因其性价比高而被认为最有可能大规模应用的生物基高分子之一。但是在实际应用中，聚乳酸存在的许多缺点如耐热性能差、脆性大、价格高
Using engineered bacteria as therapeutic agents against tumors is one of the state-of-the-art techniques emerging with the development of synthetic biology.
丁醇是重要的大宗化学品,可通过产溶剂梭菌发酵生产.拜氏梭菌对秸秆类木质纤维素水解液的耐受性较好,但对水解液中己糖和戊糖的共发酵能力有待提升.我们改进了原有TargeT
在构建工业微生物的过程中，我们需要一个能有效进行基因组规模的基因编辑的工具。通过将酿脓链球菌二型CRISPR-Cas9系统运用到大肠杆菌中，我们构建了一个靶向的、连续的多
天冬氨酸-β-半醛脱氢酶(Aspartate-β-semialdehyde dehydrogenase,ASADH)位于天冬氨酸家族氨基酸合成途径中关键节点位置,在生理方向催化天冬氨酸-β-磷酸转化为天冬氨
Cells navigate environments and regulate gene expression in response to specific signals.Engineers seek to harness this capability to reprogram cells to per