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[Purpose]Two strains named SIPI-226 arid SIPI-207 were isolated from soil samples collected in a park of Wuxi city in China.[Method] Identification was done by morphological observation,chemical analysis and nucleic acid ribotyping and 16S rRNA sequence.[Rethod]It was revealed that these two strains have 97.2-98.4% and 98.2-98.6% homology in 16S rRNA gene sequences with the related Microbispora,but differ from seven other mesophilic Microbispora rosea strains [conclution]It could be concluded that these two strains represented two new subspecies in the genus of Microbispora rosea.We designated the strains as Microbispora rosea subsp wuxiensis and Microbispora rosea subsp,yuantouzhuensis.