论文部分内容阅读
Separation of an aromatic amine is presented using a commercially available stationary phase-Chirosil RCA (+) which consists of a chemically bonded crown ether tetracarboxylic acid.To separate this amine we used mobile phases containing organic mobile phase such as methanol or acetonitrile and 0.1% HClO4 in water.Plots of ln k vs.the volume fraction of the organic modifier as well plots of ln k and vs.1/T showed a non-linear behavior which prompted us to look at non chromatographic techniques.These results prompted us to undertake non chromatographic experiments,such as infrared spectrometry and vibrational circular dichroism,in order to elucidate the particular interactions between the chiral amine and the crown ether tetracarboxylic acid.