Neurexin Regulates Lamina Neurons Axonal Tiling Through Ephrin/EPH Signaling in Drosophila

• 作 者: YaoTian;LinaLiu;WeiXie;JunhaiHan;
• 机 构: Institute of Life Sciences,the Key Laboratory of Developmental Genes and Human Disease,Southeast Uni
• 出 处: Symposium for Chinese Neuroscientists Worldwide 2014(第八届海内外华
• 发表日期: 2014年期

　　Neurexins are cell adhesion molecules involved in synapse formation and synaptic regulation.However,the initiation of Neurexin expression is much earlier than synaptogenesis.The role of Neurexin in early neural development is unknown.Here,we show that Drosophila α-Neurexin plays an important role in axonal tiling through regulating Ephrin/EPH signaling.Neurexins is expressed in developing lamina neurons,and loss of Neurexin leads to abnormal axonal tiling in lamina neurons.Using yeast two-hybrid screening,we identify that Neurexin interacts with Ephrin,a classical axon guidance molecule.We further reveal that Ephrin co-localizes with Neurexin in developing lamina neurons and Ephrin protein level is significantly reduced in nrx mutant lamina neurons.Finally,we demonstrate that Neurexins are critical for Ephrin transport to plasma membrane.Our studies reveal a important role for Neurexin in early neural development,regulating Ephrin protein transport and axonal tiling.