A transcriptome-wide in vivo snapshot of the RNA-RNA interactome in Escherichia coli

• 作 者: 刘涛 徐淞 付汉江 王江 王正 郑晓飞 李伍举
• 机 构: 军事医学科学院基础医学研究所,计算生物学中心,北京100850 军事医学科学院放射与辐射医学研究所
• 出 处: 第七届全国生物信息学与系统生物学学术大会
• 发表日期: 2016年8期
• 关键词: RNA interactome high-throughput sequencing bacteria

　　sRNAs and their targets were usually discovered by bioinformatics prediction combined with experimental verification,which was hampered by the low prediction accuracy and low-throughput.Among the 2792 sequenced microbiological genomes,only about 1000 sRNAs had been found.Furthermore,target mRNAs of only 134 sRNAs had been uncovered[1].Obviously,more sRNAs and target RNAs remained to be discovered.The chemical psoralen can intercalate into RNA helices and after irradiation with 320-400 nm light,freeze (in a helical or pseudoknot form) the uridines of RNA by covalent attachment[2]if they are in close proximity[3].