【摘 要】
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Cardiac stem cell (CSC) transplantation is thought to be responsible for improving the performance of injured heart induced by myocardial infarction (MI).However,the mechanisms involved in the migrati
【机 构】
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Cardiovascular Institute of Affiliated Hospital,Provincial Key Laboratory of Tropical Medicine,Haina
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Cardiac stem cell (CSC) transplantation is thought to be responsible for improving the performance of injured heart induced by myocardial infarction (MI).However,the mechanisms involved in the migration of activated CSCs post-MI are not well understood.In this study,CSCs were isolated from rat hearts and a cellular migration assay was performed using a 24-well transwell system.Stem cell factor (SCF) induced CSC migration in a concentration-dependent manner,which could be blocked with an SCF antibody as well as a PI3K/AKT inhibitor,Ly294002.Moreover,SCF induced the expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9 in a concentration and time-dependent manner,as measured by quantitative PCR,western blotting and gelatin zymography.Western blot analysis also showed that phosphorylated AKT was markedly increased in SCF-treated CSCs and that inhibition of SCF/c-kit signaling or phospho-AKT activity significantly attenuated the SCF-induced expression of MMP-2 and MMP-9.Thus,our results revealed that SCF mediated CSC migration at least partly via activation of PI3K/AKT/MMP-2/-9 signaling.
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