【摘 要】
：
Adoptive therapy is a promising approach for the treatment of cancers.In adoptive therapy,antigen-presenting cells (APCs) loaded with tumor specific antigens stimulate the proliferation of peripheral
【机 构】
：
National Center for International Research of Biological Targeting Diagnosis and Therapy,Guangxi Med
论文部分内容阅读
Adoptive therapy is a promising approach for the treatment of cancers.In adoptive therapy,antigen-presenting cells (APCs) loaded with tumor specific antigens stimulate the proliferation of peripheral blood mononuclear cells (PBMCs) to produce tumor antigen-specific CTLs that are transferred back to patients to kill cancer cells.However,tumor antigen-specific CTLs are difficult to get to tumor tissues,due to immune suppression in patients.Interferon-inducible protein-10 (IP-10),a powerful chemokine,attracts CTLs to tumor tissues and improves their anti-tumor activity.In this study,we developed folate-modified chitosan nanoparticles coating the human IP-10 gene (FA-CS-hIP-10),which bound to the folate receptors on hepatoma cells and promoted the expression of IP-10 to improve the activity of pMAGE-A1278-286 specific CTLs.The combination of FA-CS-hIP-10 and pMAGEA1278- 286 specific CD8+ CTLs increased the secretion of IFN-γ,inhibited tumor growth and extended the survival of nude mice with subcutaneously transplanted human hepatocellular carcinoma.Our results demonstrated that the mechanism of this novel therapy approach was involved in the inhibition of angiogenesis,inhibition of proliferation,and promotion of apoptosis of tumor cells.Our study provided a potentially novel approach for the treatment of human hepatocellular carcinoma by improving the activity of tumor antigen-specific CTLs.
其他文献
为数字化评价针芽形绿茶外形品质,建立一种针芽形绿茶外形品质智能化感官评价方法.建立针芽形绿茶外形品质评价模型.本文研究采用由摄像头、样品池、积分球、计算机和软件组成的试验装置,构建计算机视觉成像技术,采集我国12个省市针芽形绿茶产地160个样本图像的红体图像均值、绿体图像均值、蓝体图像均值等15个颜色特征参数和基于统计矩特征的平均灰度值、标准差、平滑度等6个纹理特征参数.将所收集的浙江、江苏两省针
根据田间调查的结果,采用聚集度指标法、Iwao回归分析法和Taylor幂指数法对无锡地区茶园绿盲蝽的空间分布型和理论抽样数进行分析研究.结果表明:绿盲蝽在茶园中的空间分布型呈聚集分布,聚集程度随种群密度的增加而增强.同时根据Iwao回归分析法中的理论抽样数模型确定绿盲蝽的最适理论抽样数.用α、β参数计算出绿盲蝽田间调查的理论抽样数公式为N=t2/D2〔0.923/n+1.271.通过建立理论抽样数
松阳红茶成为近几年松阳茶叶发展的新秀,为此笔者开展了松阳红茶的品种适制性研究.根据研究结果、实践经验,本文提出金观音无论从外形、汤色、香气、滋味、叶底方面还是从理化指标方面分析,品质表现优异,是10 个品种中最佳的制作松阳红茶的茶树品种.福云3号、丽早香、乌牛早、迎霜、鸠坑有性群体种也是制作松阳红茶品质较为理想的茶树品种.其次为福云6 号、龙井43等茶树品种.平阳特早品种加工松阳红茶品质一般.安吉
为了解云南省流行性乙型脑炎(乙脑)病人感染乙脑病毒(JEV)基因型状况,在云南省采集脑炎病人急性期血清标本和脑脊液标本,用RT-PCR检测JEV核酸,阳性者进行JEV-PreM/C区基因序列测定,用核苷酸同源性和系统进化分析进行基因型鉴定.从11例脑炎病人脑脊液标本中检测到JEV核酸阳性3份(JB114、JB135和DLN24),而20例脑炎病人血清标本均为阴性.这3株病毒的PreM/C区核苷酸序
在汽车、工程机械产业，因产品尺寸大、装配复杂，难以采用单边生产线，故双边装配线得以广泛应用。双边装配线平衡问题(two-sided assembly lines balancing problem, TALBP)因此受到较多关注。双边装配线平衡问题分为两类:第一类平衡问题为在限定节拍时间的前提下，尽量缩减装配线长度;第二类平衡问题为在限定装配线成对工位数目的情况下，尽量缩减工位的节拍。生产实际中为
通过涂层划擦试验比较了PVD TiCrAlN和AlTiN两种刀具涂层与基体的结合强度,利用超声辅助振动的方式,用金刚石压头对两种涂层进行不同振幅下的高频冲击试验.采用扫描电子显微镜(SEM)和3D轮廓仪等对冲击后涂层的表面破坏形式和压痕形状进行检测,比较两种涂层的抗冲击能力,分析了涂层冲击失效机理.结果表明,TiCrAlN要比AlTiN涂层与基体界面处的结合力好;涂层在受到冲击时产生侧向裂纹和径向
在高附加值的精密磨削中,金刚石砂轮的修锐是关键,但是,如何控制微米尺度的金刚石磨粒出刃状态一直依赖于现场经验.本文提出脉冲放电微去除的金刚石砂轮修锐方法,构建微脉冲放电电流和电压波形的特征化参数与砂轮结合剂微去除的关系模型,实现精密磨削中的微磨粒空间出刃的智能化控制.首先,将在线采集的脉冲放电波形特征化为脉冲放电参数(脉冲峰值电流Ie和脉冲持续时间τe).然后,根据脉冲放电参数计算出单个放电坑的去
环氧合酶-1(COX-1)是COX家族中组成性表达的成员.为探讨COX-1在急性肝损伤中的作用和机制,通过注射四氯化碳(CCl4)诱导野生型或COX-1敲除小鼠的急性肝损伤,研究各种损伤表征的变化.结果表明,完全或部分敲除COX-1基因、使用药物抑制COX-1 酶活性均可恶化四氯化碳造成的急性肝损伤,主要表现在:恶化的肝脏组织学和血清中转氨酶的变化;通过CYP2E1介导的氧化压力的恶化;升高的肝脏
Folate-chitosan nanoparticles (FA-CS) were loaded with mouse interferon-inducible protein-10 (IP-10) plasmid DNA to generate FA-CS-IP-10,which was used for immunotherapy in hepatocellular carcinoma (H
We previously found that T cell immunoglobulin and mucin domain protein 3 (Tim-3) signaling contributed to intestinal homeostasis while the underlying mechanisms remain unclear.Here we demonstrate tha