Gene Order Rearrangement of the M Gene in Rabies Virus Leads to Slower Replication

• 作 者: Xian-FengYang[1]Jiao-JiaoPeng[1]Hong-RuLiang[2]You-TianYang[1]Yi-FeiWang[1]Xiao-WeiWu[3]Yong-WenLuo[1]Xiao-FengGuo[1]
• 机 构: College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China
• 出 处: 第四届人畜共患病学术研讨会、2014年中国狂犬病年会、中国畜牧兽医学会兽医公共卫生学分会第四次学术研讨会
• 发表日期: 2014年期

　　The matrix protein (M) is one of only five genes in the RV genome and is an important multifunctional protein.Besides to allow for the release of newly replicated virions pairing with G,the M protein also functions in virus replication,pathogenicity,and host cell apoptosis.The goal of present study is to generate recombinant viruses with M gene rearranged,thus laying the foundation for further exploring what will happen when the gene for M is relocated on the RV single-strand RNA.We used rHEP-Flury,an attenuated virus that remains virulent for less than three-days in sucking mice,to reshuffle the M gene,using an approach that leaves the other viral nucleotide sequence intact.Two viruses with translocated M genes (NIM2 and NIM4) were recovered from each of the rearranged cDNAs.whose gene order is 3 -N-M-P-G-L-5 and 3 -N-P-G-M-L-5 respectively.The growth dynamics of these viruses showed slower replication than the wild-type virus in multiple-step growth curves,but they can grow to a comparable titer in tests of single-step growth curves.Further experimentation with these rearranged viruses will provide insights into the relationships between genome structure and virus phenotypes.