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Despite its high resolution power, sensitivity, precision and practicability, high-performance liquid chromatographic (HPLC) analysis of biological samples like blood, serum, plasma, urine, milk, lymphatic fluids, liquor, faeces or tissue homogeuates is restricted by the pretreatment and processing of such highly complex matrices.Often the sample preparation represents the weakest chain segment in an analytical HPLC procedure as it still involves many elaborate, manual and thus error-prone and time consuming work-up steps.To solve these problems, direct injection supports and on-line chromatographic techniques have been developed in HPLC.The mixed functional silica having β-cyclodextrin and diol is an important kind of direct injection supports.