Morroniside prevents peroxide-induced apoptosis by induction of endogenous glutathione in human neur

• 作 者: WenWANG[1]Wen-TingHUANG[2]LinLi[1]Hou-XiAI[1]Fang-LingSUN[2]CiLIU[1]YiAn[2]
• 机 构: Department of Pharmacology, Xuan-Wu Hospital of Capital Medical University, Key Laboratory for neuro
• 出 处: 中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
• 发表日期: 2007年期

　　(1) Morroniside belongs to an extensive group of natural iridorid glycosides.In the present study,using human neuroblastoma SH-SY5Y cells,we have investigated the protective effects of this compound on modifications in endogenous reduced glutathione (GSH),intracellular oxygen species (ROS) and apoptotic death on H2O2-mediated cytoxicity.(2) Incubation of cells with morroniside led to a significant dose-dependent elevation of cellular GSH accompanied by a marked protection against H2O2-mediated toxicity.Morroniside at 1-100 μmol/L inhibited the formation of ROS and the activation of caspase-3 and 9,and the upregulation of Bcl-2,whereas no significant change occurred in Bax levels.(3) Our results indicated that the anti-oxidative and anti-apoptotic properties render this natural compound potentially protective against H2O2-induced cytotoxicity.(4) This study suggested that intracellular GSH appeared to be an important factor in morroniside-mediated cytoprotection against H2O2-toxicity in SH-SY5Y cells.