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The complete nucleotide sequence of Tomato 4spermy Virus (TAV) isolated from chrysanthemum in Henan province (TAV-Henan) was determined.TAV contains a tripartite genome of messenger plus-sense single-stranded RNAs designated RNA 1, 2 and 3.The genome of TAV RNA1, 2 and 3 were 3408, 3095 and 2222 nucleotides in length, and contained five open reading frames (ORFs).