BmICE-2 is a novel pro-apoptotic caspase involved in apoptosis in the silkworm, Bombyx mori

• 作 者: Hua-ShanYi;Cai-XiaPan;ChunPan;JuanSong;Yan-FenHu;LaWang;Min-HuiPan;ChengLu;
• 机 构: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China
• 出 处: 中国”西南地区农业动物种质资源挖掘与创新利用“学术研讨会
• 发表日期: 2014年期

　　In this study we identified a potential pro-apoptotic caspase gene,Bombyx mori(B.mori)ICE-2(BmICE-2)which encoded a polypeptide of 284 amino acid residues,including 169QACRG173 sequence which surrounded the catalytic site and contained a p20 and a p10 domain.BmICE-2 expressed in Escherichia coli(E.coli)exhibited high proteolytic activity for the synthetic human initiator caspase-9 substrates Ac-LEHD-pNA,but little activity towards the effector caspase-3 substrates Ac-DEVD-pNA.When BmICE-2 was transiently expressed in BmN-SWU1 silkwormB.