Bombyx mori nucleopolyhedrovirus ORF79 is a per os infectivity factor associated with the PIF comple

• 作 者: Zhan-QiDong;JunZhang;Xue-MeiChen;QianHea;Ming-YaCao;LaWang;Hai-QingLi;Wen-FuXiao;Cai-XiaPan;ChengLu;Min-HuiPan;
• 机 构: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China
• 出 处: 中国”西南地区农业动物种质资源挖掘与创新利用“学术研讨会
• 发表日期: 2014年期

　　Bombyx mori nucleopolyhedrovirus(BmNPV)ORF79(Bm79)encodes an occlusion-derived virus(ODV)-specific envelope protein,which is a homologue of the per os infectivity factor 4(PIF4)of Autographa californica multiple nucleopolyhedrovirus(AcMNPV).To investigate the role of ORF79 in the BmNPV life cycle,a Bm79 knockout virus(vBm Bm79KO)was constructed through homologous recombination in Escherichia coli.Viral DNA replication,budded virus(BV)production and polyhedra formation were unaffected by the absence of BM79.