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Aims To investigate the effect of advanced glycation end products (AGEs) on contractile activity of rat colon and its possible mechanisms.Methods Contractile responses of colonic smooth muscle strips from male SD rats were recorded using a polyphysiograph.Colonic smooth muscle cells were isolated and identified by immunofluorescence.Changes of intracellular calcium concentration ([Ca2+Ji) in response to AGEs (150μg/ml) was measured using Fluo3/AM and confocal laser scanning microscopy.The PKC inhibitor and activator were used to examine the role of PKC in the responses to AGEs.Immunoprecipitation and Western blotting were used to examine the phosphorylation of type 3 InsP3 receptors (InsP3R3) in colonic smooth muscle cells.