【摘 要】
：
With the completion of the human Encyclopedia of DNA Elements (ENCODE) project, an arduous task is to elucidate the function of the vast number of regulatory DN
【出 处】
：
2015 Genomics Frontiers Symposium(2015基因组学前沿研讨会)
论文部分内容阅读
With the completion of the human Encyclopedia of DNA Elements (ENCODE) project, an arduous task is to elucidate the function of the vast number of regulatory DNA elements identified in the human genome.There are millions of regulatory DNA elements in the human genome, such as enhancers (activators), silencers (repressors), promoters, insulators, and locus control regions.The clustered regularly-interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) programed with a synthetic single-guide RNA (sgRNA) emerges as a method for genome editing in virtually any organisms.Here we report an efficient technology of targeted DNA fragment inversion in human and mouse genomes by CRISPR/Cas9 with two sgRNAs.
其他文献
比目鱼肌肌瓣的相关解剖概念人体解剖形态血供构筑形式临床应用的历史和现状肌瓣临床应用开始达百年.比目鱼肌瓣应用 19661980以来得到深入研究近年来各基层医院有广泛应用报
目录CONTENTS01中国糖尿病管理现状和存在的问题02三诺血糖管理系统是糖尿病全程智能化管理的优选01中国糖尿病管理现状和存在的问题
Disclosure Acted as a consultant / speaker for Amylin Pharmaceuticals LLC,Novartis, Roche, Sanofi, AstraZeneca and GI Dynamics Received grants in support from P
学术前沿:探讨糖尿病精准医疗2017AACE年会,Judith Fradkin博士发表演讲:《糖尿病精准医疗:我们到哪了?》精准药物设计,是精准医疗的技术支撑精准医疗技术支撑药物基因组学精
Objective:Coronary heart disease arising from atherosclerosis is a leading cause of cardiogenic death worldwide.Uncoupling protein 2 (UCP2) protects against mit
Objective:Both Innative and adaptive immune are involved in blood pressure regulation.Lymphocyte depletion confers protection against Ang Ⅱ-induced hypertensio
Recent studies suggest that high fructose (HF) diet induces salt-sensitive hypertension but the underlying mechanism largely remains elusive.We hypothesize
Enterococcus faecalis is a Gram-positive bacterium that natively colonizes the human gastrointestinal tract and opportunistically causes life-threatening infect
Many enhancers regulate their target genes via long distance interactions.High-throughput experiments like ChIA-PET have been developed to map such interactions
Objective:Hydronephrosis is characterized by substantial loss of tubules and affects renin secretion in the kidney.However, whether alterations of angiotensin c