ABCG2 monoclonal antibody in combination with NPs-PTX in vitro inhibited multiple myeloma cancer ste

• 作 者: YANGCui-ping[1]CHENBao-an[2]XIONGfei[3]DOUJun[1]LIUYun-jin[1]CHENJun-song[1]GUNing[3]
• 机 构: Department of Pathogenic Biology and Immunology, Medical School, Southeast University
• 出 处: 第八届全国难治性白血病学术研讨会、第四届全国难治性淋巴瘤学术研讨会、第四届全国多发性骨髓瘤学术研讨会、第五届钟山国际MD
• 发表日期: 2012年期

　　Objective To detect the inhibitive effect on multiple myeloma (MM) cancer stem cells(CSCs) by ABCG2 monoclonal antibody (McAb) in combination with Fe3O4 nanoparticle-paclitaxel(NPs-PTX) in vitro.Mothods MM CSCs were sorted from human MM cell lines NCI-H929 and RPMI8226 through the immune magnetic beads sorting method.Growth inhibitive effect was detected by CCK-8 assay.Effects of cell cycles and apoptosis were de tected by flow cytometry.Results The proliferation of NCI-H929 and RPMI8226 cells were suppressed after treat ment with ABCG2 McAb in combination with NPs-PTX for 24 hours.There is statistical significant difference between the combined utilization of ABCG2 McAb and NPs-PTX group in contrast to the utilization of NPs-PTX group alone.