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Sisal is the most important fiber crop in tropical and subtropical areas in China and theworld.Zebra disease is a serious threat to the main cultivar Agave hybrid No.11648 (H.11648) worldwide.To select germplasm materials with zebra disease resistance for breeding, the fluorescent amplified fragment length polymorphism(AFLP)technique was used to make a cluster analysis of the genetic relationships of 40 sisal genotypes grown in China, and Phytophthora nicotianae was used to inoculate the 40 genotypes to identify their resistance to zebra disease.