【摘 要】
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In order to establish a proper two-dimensional gel electrophoresis(2-DE)protocol for proteomic study of buckwheat seeds,Miaoqiao No.1 seed was taken as expe
【机 构】
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CollegeofLifeScience,SichuanAgriculturalUniversity,Ya'an,625000,China
【出 处】
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全国农业生物化学与分子生物学第十六届学术研讨会
论文部分内容阅读
In order to establish a proper two-dimensional gel electrophoresis(2-DE)protocol for proteomic study of buckwheat seeds,Miaoqiao No.1 seed was taken as experimental material,seed protein sample of Miaoqiao No.l prepared by three methods.Two-dimensional electrophoresis was optimized in regard to protein extraction,the suitable concentration of protein per IPG gel,repetitions of experiment and SDS-PAGE parameters.The results showed the optimized system includes:total proteins of sample extracted using Phenolic extraction method,separating the proteins with 17 cm pH 4~7 IPG strips,800 μg and focusing time of 70 000 Vh.About 1193 spots can be detected,and these proteins mainly distributed in the pH 4~7 range.With good separation of the basic protein,this will be helpful to proteomic research in buckwheat seed.
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